Pesquisa | SciELO

Resumo Um macho de Pteronura brasiliensis (Carnivora: Mustelidae) foi encontrado morto às margens do Rio Negro, no Pantanal do estado de Mato Grosso do Sul, município de Aquidauana. Dois carrapatos encontrados aderidos à sua pele foram identificados, morfologicamente, como uma ninfa de segundo ínstar de Ornithodoros rostratus (Argasidae) e um macho de Amblyomma sculptum (Ixodidae). Para complementar a identificação morfológica, esses espécimes de carrapatos foram submetidos à extração de DNA e testados por meio de ensaios de PCR, para confirmar a identidade molecular dos espécimes. Além disso, os extraídos dos carrapatos foram testados e revelaram-se negativos nos ensaios de PCR para todos os patógenos testados. Também foram examinados 30 lotes, compostos por 174 indivíduos de O. rostratus, depositados na Coleção Acari do Instituto Butantan, e encontramos material de quatro estados brasileiros, incluindo-se um lote contendo 2 machos e 2 fêmeas de Aquidauana, estado de Mato Grosso do Sul (MS), coletado do solo. Este foi, portanto, o primeiro registro de O. rostratus parasitando P. brasiliensis e o primeiro registro de localidade (Aquidauana). Da mesma forma, A. sculptum é comumente encontrado no Pantanal e relatado aqui, pela segunda vez, parasitando a ariranha, hospedeiro pouco estudado em relação a ectoparasitas.

Abstract A male of Pteronura brasiliensis (Carnivora: Mustelidae) was found dead on the banks of the Rio Negro, in the Pantanal wetlands of Mato Grosso do Sul state, Aquidauana municipality. Two ticks found attached to its skin were morphologically identified as a second-instar nymph of Ornithodoros rostratus (Argasidae) and a male of Amblyomma sculptum (Ixodidae). In order to complement the morphological identification, these tick specimens were subjected to DNA extraction, and tested using PCR assays to confirm the molecular identity the specimens. Also, the tick DNA samples were tested and were negative in the PCR assays for all the pathogens tested. We also examined 30 batches, consisting of 174 individuals of O. rostratus deposited in the Acari Collection of the Butantan Institute, and we found material from four Brazilian states, including one batch containing 2 males and 2 females from Aquidauana, of Mato Grosso do Sul state, collected from the soil. This was therefore the first record of O. rostratus parasitizing P. brasiliensis and the first locality record (Aquidauana). Likewise, A. sculptum is commonly found in the Pantanal and is reported here for the second time parasitizing the giant otter, which is a host little studied regarding the ectoparasites.

https://doi.org/10.1590/s1984-29612024003

Performance Evaluation of Geometrically Different Pediatric Arterial Cannulae in a Pediatric Cardiopulmonary Bypass Model
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Carvalho, Gabriela B. de O.

; Caneo, Luiz Fernando

; Matte, Gregory

; Cruz, Caio Henrique de A.

; Silva, Everton Neri da

; Carletto, Luciana P.

; Castro, Ana Vitória C. X. de

; Silva, Betina G. Madueño

; Policarpo, Valéria C.

; Cestari, Idágene A.

; Jatene, Fabio B

; Jatene, Marcelo Biscegli

Brazilian Journal of Cardiovascular Surgery

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2024, Volume 39 Nº 1 elocation e20230110

Resumo: En | Texto: En | PDF: En

ABSTRACT Objective: To define a reference chart comparing pressure drop vs. flow generated by a set of arterial cannulae currently utilized in cardiopulmonary bypass conditions in pediatric surgery. Methods: Cannulae from two manufacturers were selected considering their design and outer and inner diameters. Cannula performance was evaluated in terms of pressure drop vs. flow during simulated cardiopulmonary bypass conditions. The experimental circuits consisted of a Jostra HL-20 roller pump, a Quadrox-i pediatric oxygenator (Maquet Cardiopulmonary AG, Rastatt, Germany), and a custom pediatric tubing set. The circuit was primed with lactated Ringer’s solution only (first condition) and with human packed red blood cells added (second condition) to achieve a hematocrit of 30%. Cannula sizes 8 to 16 Fr were inserted into the cardiopulmonary bypass circuit with a “Y” connector. The flow was adjusted in 100 ml/min increments within typical flow ranges for each cannula. Pre-cannula and post-cannula pressures were measured to calculate the pressure drop. Results: Utilizing a pressure drop limit of 100 mmHg, our results suggest a recommended flow limit of 500, 900, 1400, 2600, and 3100 mL/min for Braile arterial cannulae sizes 8, 10, 12, 14, and 16 Fr, respectively. For Medtronic DLP arterial cannulae sizes 8, 10, 12, 14, and 16 Fr, the recommended flow limit is 600, 1100, 1700, 2700, and 3300 mL/min, respectively. Conclusion: This study reinforces discrepancies in pressure drop between cannulae of the same diameter supplied by different manufacturers and the importance of independent translational research to evaluate components’ performance.

https://doi.org/10.21470/1678-9741-2023-0110

Elaboración y rizobacterias en el control biológico de Phythopthora capsici en cultivares de Capsicum annuum
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Leos-Escobedo, Lucio

; García-Carrillo, Mario

; Delgadillo-Martínez, Julian

; Valenzuela García, Ana Alejandra

; Angulo-Castro, Azareel

; Preciado-Rangel, Pablo

; Rueda Puente, Edgar Omar

Biotecnia

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Abr 2022, Volume 24 Nº 1 Páginas 30 - 37

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen La reconversión de la agricultura, demanda alternativas sustentables entre los que destacan el uso de hongos rizosféricos =hongos micorrízicos arbusculares (HMA) y las rizobacterias promotoras del crecimiento vegetal (Rpcv). El objetivo general de la presente investigación consistió en evaluar consorcios de hongos micorrízicos y rizobacterias como control biológico vs Phythopthora capsici, en cultivares de chile (Capsicum annuum L.). Se evaluaron 23 genotipos de chiles. Los HMA que conformaron el consorcio fueron: seis cepas de Rhizophagus intraradices. Asimismo, se incluyeron cuatro Rpcv, dos del género Pseudomonas, Acinetobacter guillouiae y Aeromona caviae. Los tratamientos de estudio fueron cuatro: T1= Testigo (Sin aporte nutrimental (agua)), T2= Consorcio = (mezcla) de los seis HMA, T3= Consorcio de seis HMA más el consorcio de cuatro Rpcv, T4= Fertilización química a base una solución nutrimental. Se incrementó la resistencia de plantas al ataque de P. capsici, resultando en una reducción en su capacidad de ataque, en plantas de 23 cultivares de chile. Los cultivares de chiles tipo Pasilla y tipo Guajillo, presentaron la mayor resistencia al ataque de P. capsici, cuando fueron inoculados con HMA+Rpcv, mientras que los chiles tipo Serrano, Puya y Jalapeño, fueron los de mayor susceptibilidad al ataque del hongo fitopatógeno.

Abstract The reconversion of agriculture demands sustainable alternatives, among which the use of rhizospheric fungi = arbuscular mycorrhizal fungi (AMF) and plant growth-promoting rhizobacteria (Rpcv) stand out. The general objective of the present investigation was to evaluate consortiums of mycorrhizal fungi and rhizobacteria as biological control vs Phythopthora capsici, in cultivars of 23 genotypes of chili peppers. The AMF that made up the consortium were: six strains of Rhizophagus intraradices. Likewise, four strains of Rpcv, three of the Pseudomonas genus, Acinetobacter guillouiae and Aeromona caviae. The study treatments were four: T1 = Control (No nutritional contribution (water)), T2 = Consortium = (mixture) of the six AMF, T3 = Consortium of six AMF plus the consortium of four Rpcv, T4 = Chemical fertilization based on a nutrient solution. The plants resistance to the P. capsici attack increased, resulting in a reduction in the fungus attack capacity in plants of 23 cultivars of chile. The Pasilla-type and Guajillo-type chili cultivars presented the highest resistance to the attack of P. capsici, when inoculated with AMF + Rpcv, while the Serrano, Puya and Jalapeño-type chilies were the ones with the highest susceptibility to the attack of the phytopathogenic fungus.

https://doi.org/10.18633/biotecnia.v24i1.1490 40 downloads

Recuperación de poblaciones de tortuga verde y sus interacciones con la duna costera como línea base para una restauración ecológica integral
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Guzmán-Hernández, Vicente

; Monte-Luna, Pablo del

; López-Castro, Melania

; Uribe-Martínez, Abigail

; Huerta-Rodríguez, Patricia

; Gallegos-Fernández, Sandra A.

; Azanza-Ricardo, Julia

; Martínez-Portugal, Rosa C.

; Barragán-Zepeda, Ana K.

; Quintana Pali, Guadalupe P.

; Martín-Viaña, Yanet F.

; Gómez-Ruíz, Pilar A.

; Acosta-Sánchez, Héctor H.

; López-Hernández, Martha

; Castañeda-Ramírez, D. Gerardo

; Ortíz, Antonio

; Cuevas, Eduardo

Acta botánica mexicana

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2022, Nº 129 elocation e1954

Resumo: Es En | Texto: Es En | PDF: Es | PDF: En

Resumen: Antecedentes y Objetivos: La restauración ecológica se ha centrado en los componentes físicos y vegetales de los ecosistemas, y ha dejado de lado a la fauna silvestre y sus interacciones. Chelonia mydas es un macroherbívoro en peligro de extinción que anida en playas de México y Cuba, e influye en la vegetación costera con aportaciones de materia orgánica. Los objetivos de este estudio fueron analizar su recuperación poblacional, un índice del verdor de la vegetación de duna, así como la cantidad de materia orgánica estimada de sus nidadas antes y después de la implementación de una Norma Oficial Mexicana. Métodos: Se analizaron datos de entre dos y tres décadas de monitoreo biológico en 16 playas de anidación de Chelonia mydas; a partir de ellos se realizaron tres niveles de análisis de indicadores demográficos, la aportación de materia orgánica de las tortugas en la playa y el vigor vegetal a partir de sensores remotos, además de aplicar los estadísticos pertinentes. Resultados clave: Hubo un crecimiento exponencial del número de anidaciones de tortuga verde (entre 12 y 14% anual), asociado con pulsos de reclutamiento poblacional en los años 2000 y 2008. El aporte de materia orgánica a la playa fue significativamente mayor después de un cambio en la estrategia para manejo de las nidadas en México, y se detectó un incremento en el verdor de la vegetación de duna en zonas de anidación alta de Chelonia mydas. Conclusiones: El incremento de las poblaciones de tortuga verde lleva a reflexionar acerca de las implicaciones que tiene la recuperación de especies de fauna sobre otras comunidades en los ecosistemas que habitan, lo que demanda el análisis de los paradigmas actuales y la adaptación de estrategias que atiendan sus necesidades de manejo, procurando las condiciones de integridad ecológica de sus hábitats.

Abstract: Background and Aims: Ecological restoration has focused on the physical and vegetation components of ecosystems, leaving aside studies on wild fauna and their interactions. Chelonia mydas is an endangered macroherbivore that nests on sandy beaches of Mexico and Cuba, and influences the coastal vegetation with organic matter contributions. The objectives of this study were to analyze its population recovery, a greenness index for dune vegetation, and the amount of organic matter estimated from its clutches before and after the implementation of an official Mexican norm. Methods: Biological data obtained from two and three decades of monitoring 16 Chelonia mydas nesting beaches were analyzed; based on these, three levels of demographic indexes were carried out, the contribution of organic matter by marine turtles to the beach and the vegetal vigor using remote sensing, besides implementing the pertinent statistical analysis. Key results: There was an exponential increase of the green turtle nests (between 12 and 14% per year), associated with population recruitment pulses in 2000 and 2008. The contribution of organic matter to the beach was significantly higher after a change in clutches management in Mexico, and an increase in the dune vegetation greenness was detected in high Chelonia mydas nesting zones. Conclusions: The increase of the green turtle populations invites to reflex about the implications of the recovery of species of fauna have on other communities in the ecosystems they inhabit, demanding the analysis of the actual paradigms and the adaptation strategies that attend their management needs, procuring the ecological integrity conditions of their habitats.

https://doi.org/10.21829/abm129.2022.1954

Comparando o desempenho de dois analisadores de massas (FT-ICR MS & LTQ MS): uma aula experimental sobre análise de compostos orgânicos
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Lyrio, Marcos V. V.

; Feu, Amanda E.

; Motta, Larissa C.

; Figueroa, Laura P. R.

; Boasquevisque, Leonardo

; Pinto, Roberta T.

; Rosa, Ana Caroline L.

; Arantes, Heryson T. L. de

; Bottocim, Ramon R. T.

; Fonseca, Victor da R.

; Santos, Nayara A.

; Lacerda Jr., Valdemar

; Castro, Eustaquio V. R.

; Romão, Wanderson

Química Nova

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2022, Volume 45 Nº 4 Páginas 455 - 465

Resumo: Pt | Texto: Pt | PDF: Pt

Mass spectrometry (MS) is a high-performance analytical technique that allows a measurement of the mass/charge (m/z) ratio of compounds present in a sample. MS is able to separate two m/z ions with determined values (mass resolution), being an important characteristic, especially in the analysis of biochemical components and complex mixtures. Mass analyzers define the resolving power and accuracy of different types of mass spectrometers. Herein, the performance of two mass analyzers (Fourier Transform Ion Cyclotronic Resonance (FT-ICR) and quadrupole ion trap (LTQ)) was tested on low (Mw = 165-679 Da) and high (Mw = 783-8.58 kDa) molecular weight molecules (Phenacetin, Procaine, Benzocaine, Caffeine anhydrous, Lidocaine, two metal porphyrin standards, the first cobalt and the second vanadium, and two protein standards, Ubiquitin and Beauvericin), and finally, for an oil sample. LTQ-MS and FT-ICR-MS had similar performance and high reproducibility. However, the mass accuracy and resolution power of the FT-ICR (error lower than 5 ppm and RP higher than 400,000) was higher, being therefore, it was more suitable for the analysis of proteins and oil, for instance. On the other hand, the CID analysis via LTQ demonstrated its applicability in fragmentation experiments, being able to determinate the chemical connectivity of organic compounds in non-complex mixtures.

https://doi.org/10.21577/0100-4042.20170832

Identification of cryptic species in allopatric populations of Hypostomus tietensis (Siluriformes: Loricariidae) through cytogenetics analyses
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Paula, Gabriela B. N. de

; Gavazzoni, Mariane

; Zawadzki, Cláudio H.

; Fernandes, Carlos A.

; Portela-Castro, Ana L. B.

; Lui, Roberto L.

; Margarido, Vladimir P.

Neotropical Ichthyology

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2022, Volume 20 Nº 2 elocation e210158

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Hypostomus é o gênero mais especioso de Hypostominae, composto por várias espécies com uma alta variação tanto morfológica, como no padrão de coloração intraespecífica, tornando sua identificação uma questão complexa. Uma das espécies com identificação complexa é Hypostomus tietensis, a qual foi descrita a partir de um único espécime, resultando em incertezas sobre o seu padrão de cor e identificação. Para auxiliar nesse contexto, análises citogenéticas foram realizadas em três populações putativas de H. tietensis da bacia do Alto rio Paraná, sendo uma delas da localidade tipo. As três populações apresentaram diferenças citogenéticas consideráveis, com 2n = 72 cromossomos para a população da localidade tipo e as demais com 2n = 76. RONs terminais foram detectadas (Ag- e FISH-DNAr 18S), sendo simples para a população da localidade tipo (par acrocêntrico 23, braço longo) e do rio Pirapó (par subtelocêntrico 11, braço curto) e múltiplas para rio Do Campo (pares subtelocêntricos 11 e 12, braço curto e longo, respectivamente), confirmado pela FISH-DNAr 18S. O bandamento C foi eficiente em diferenciar a população da localidade tipo das demais. Os dados citogenéticos revelaram que as populações dos rios Pirapó e do rio Do Campo, embora tratadas até agora como Hypostomus aff. tietensis, representam uma espécie críptica, e que análises morfológicas são necessárias para diferenciar e descrever esta nova espécie.

Abstract Hypostomus is the most specious genus of Hypostominae, composed of several species with high intraspecific morphological and color pattern variation, making their identification a complex issue. One of the species with problematic identification is Hypostomus tietensis that was described from a single specimen, resulting in uncertainties about its color pattern and correct identification. To assist in this context, cytogenetic analyzes were carried out in three putative populations of H. tietensis from the Upper Paraná River basin, one of them from the type locality. The three populations showed considerable cytogenetic differences, with 2n = 72 chromosomes for the population from the type locality and 2n = 76 chromosomes for the others. Terminal NORs were detected (Ag- and 18S rDNA-FISH), being simple for the type locality population (acrocentric pair 23, long arm) and the Pirapó River (subtelocentric pair 11, short arm), and multiple for Do Campo River (subtelocentric pairs 11 and 12, short and long arm, respectively). C-banding was efficient in differentiating the type locality population from the others. Cytogenetic data revealed that populations from Pirapó and Do Campo rivers, although treated until now as Hypostomus aff. tietensis, represent a cryptic species, and those morphological analyses are necessary to differentiate and for describing this new species.

https://doi.org/10.1590/1982-0224-2021-0158

Do biofertilizers affect nodulation ability and pod production in peanut genotypes?
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JULIÃO, ANA KELLY S.

; LUZ, LUCAS N. DA

; GADELHA, MARYSSOL T.

; OLIVEIRA, MATHEUS L.

; SILVEIRA, MARIA V.S.

; CASTRO, SAMUEL F.A.O.

; BARROS, LENIN P.

Anais da Academia Brasileira de Ciências

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2022, Volume 94 Nº 2 elocation e20201163

Resumo: En | Texto: En | PDF: En

Abstract Peanuts are an important legume for the Northeastern Brazilian market, but their production in this region is low. The present study aimed to evaluate the effect of biofertilizer doses on peanut nodulation and production components, to define the best dose and genotype. The experiment was conducted in a completely randomized block design (DBC) in a 3 x 3 factorial arrangement, with three replications. The treatments consisted of two evaluation factors: 1) Fertilization via different doses of organic fertilizer applied to the substrate (D1 = 0mL; D2 = 500mL; and D3 = 1000mL); and 2) Peanut genotypes (BR-1, UNI43 and UNI08). The following traits were assessed: number of nodules per plant (NNP), number of mature pods (NVM), pod mass per plant (MVP), seed mass per plant (MSP) and root length (CR). Peanut production is affected by fertilization via bovine biofertilizer, mainly for the components NVM, MVP and MSP. Besides, nodulation is a likely conditioner. The 1000mL dose proved to be the best treatment for the traits analyzed, and UNI08 accession, the most responsive material.

https://doi.org/10.1590/0001-3765202220201163

Efecto de la aplicación de ácido naftalén acético sobre el enraizamiento de estacas leñosas de Croton urucurana Baill., Tabernaemontana catharinensis A. DC. y Paullinia elegans Cambess
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Biganzoli, M

; Castro, D. C

; Ghío, N

; Gabriel, P. M

; Álvarez, N

; Buyatti, M. A

Fave. Sección ciencias agrarias

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Jun 2021, Volume 20 Nº 1 Páginas 189 - 203

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

ABSTRACT The objective of this work was to evaluate the rooting potential of “Sangre de Drago” (SD: Croton urucurana Baill.), “Horquetero” (TC: Tabernaemontana catharinensis A. DC.) and “Ojo de muñeca” (PE: Paullinia elegans Cambess.) cuttings by immersion in 1-Naphthaleneacetic acid (NAA). Two hundred cuttings without leaves per species were collected and immersed in 0, 1000, 2500 and 3500 ppm of NAA (for SD and TC) and 0, 500, 1000 and 2500 ppm of NAA for PE. At the end of the rooting period the following variables were registered on each cutting; the presence of roots, diameter at the base of the cutting (DAC) and rooting quality. Rooting probability was highest (0.76) for NAA than control (0.36) on SD cuttings. The rooting probability was very low on PE regardless of the NAA dose (0.13) but was directly proportional to the DAC. Root formation was not observed on TC, regardless of the NAA dose nor DAC. Rooting quality was unaffected by NAA dose but highly related to DAC in SD.

RESUMEN El objetivo de este trabajo fue evaluar el enraizamiento de estacas de Croton urucurana Baill., Tabernaemontana catharinensis A. DC. y Paullinia elegans Cambess conocidas en Argentina como “Sangre de drago” (SD), “Horquetero” (TC) y “Ojo de muñeca” (PE), respectivamente. Se recolectaron 200 estacas leñosas por especie; una vez defoliadas se aplicó ácido naftalén acético (ANA) en las siguientes dosis: 0, 1000, 2500 y 3500 mg.L-1 en SD y TC y 0, 500, 1000 y 2500 mg.L-1 en PE. Se evaluó la formación y calidad de raíces y el diámetro de las estacas a la altura del cuello (DAC). En SD las dosis de ANA ≥1000 mg.L-1 aumentaron la probabilidad de enraizamiento de 0,36 a 0,71. En PE hubo baja probabilidad de enraizamiento (0,13) independientemente de la dosis de ANA, pero aumentó en función del DAC. No se observó formación de raíces en TC. La calidad de enraizamiento no fue afectada por la diferentes dosis de ANA, pero sí el DAC en SD.

23 downloads

Consenso de hemofilia en México
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López-Arroyo, José L.

; Pérez-Zúñiga, Juan M.

; Merino-Pasaye, Laura E.

; Saavedra-González, Azucena

; Alcivar-Cedeño, Luisa María

; Álvarez-Vera, José Luis

; Anaya-Cuellar, Irene

; Arana-Luna, Luara L.

; Ávila-Castro, David

; Bates-Martín, Ramón A.

; Cesarman-Maus, Gabriela

; Chávez-Aguilar, Lénica A.

; Peña-Celaya, José A. de la

; Espitia-Ríos, María E.

; Estrada-Domínguez, Patricia

; Fermín-Caminero, Denisse

; Flores-Patricio, Willy

; García Chávez, Jaime

; García-Lee, María T.

; González-Pérez, María del Carmen

; González-Rubio, María del Carmen

; González-Villareal, María Guadalupe

; Ramírez-Moreno, Fabiola

; Hernández-Colin, Ana K.

; Hernández-Ruiz, Eleazar

; Herrera-Olivares, Wilfrido

; Leyto-Cruz, Faustino

; Loera-Fragoso, Sergio

; Martínez-Ríos, Annel

; Miranda-Madrazo, María R.

; Morales-Hernández, Alba

; Nava-Villegas, Lorena

; Orellana-Garibay, Juan J.

; Palma-Moreno, Orlando G.

; Paredes-Lozano, Eugenia P.

; Peña-Alcántara, Paula

; Pérez-Lozano, Uendy

; Pichardo-Cepín, Yayra M.

; Reynoso-Pérez, Ana Carolina

; Rodríguez-Serna, Mishel

; Rojas-Castillejos, Flavio

; Romero-Rodelo, Hilda

; Ruíz-Contreras, Josué I.

; Segura-García, Adela

; Silva-Vera, Karina

; Soto-Cisneros, Paulina M.

; Tapia-Enríquez, Ana L.

; Tavera-Rodríguez, Martha G.

; Teomitzi-Sánchez, Óscar

; Tepepa-Flores, Fredy

; Valencia-Rivas, María D.

; Valle-Cárdenas, Teresa

; Varela-Constantino, Ana

; Javier-Morales, Adrián

; Martínez-Ramírez, Mario A.

; Tena-Cano, Sergio

; Terrazas-Marín, Ricardo

; Vilchis-González, Shendel P.

; Villela-Peña, Atenas

; Mena-Zepeda, Verónica

; Alvarado Ibarra, Martha

Gaceta médica de México

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2021, Volume 157 Supl. 1 Páginas S1 - S37

Resumo: En Es | Texto: En Es | PDF: En | PDF: Es

Abstract Hemophilia is a hemorrhagic disorder with a sex-linked inherited pattern, characterized by an inability to amplify coagulation due to a deficiency in coagulation factor VIII (hemophilia A or classic) or factor IX (hemophilia B). Sequencing of the genes involved in hemophilia has provided a description and record of the main mutations, as well as a correlation with the various degrees of severity. Hemorrhagic manifestations are related to levels of circulating factor, mainly affecting the musculoskeletal system and specifically the large joints (knees, ankles and elbows). This document is a review and consensus of the main genetic aspects of hemophilia, from the inheritance pattern to the concept of women carriers, physiopathology and classification of the disorder, the basic and confirmation studies when hemophilia is suspected, the various treatment regimens based on infusion of the deficient coagulation factor as well as innovative factor-free therapies and recommendations for the management of complications associated with treatment (development of inhibitors and/or transfusion transmitted infections) or secondary to articular hemorrhagic events (hemophilic arthropathy). Finally, relevant reviews of clinical and treatment aspects of hemorrhagic pathology charachterized by acquired deficiency of FVIII secondary to neutralized antibodies named acquired hemophilia.

resumen está disponible en el texto completo

https://doi.org/10.24875/gmm.m20000451 305 downloads

10.

Persistent symptoms and decreased health-related quality of life after symptomatic pediatric COVID-19: A prospective study in a Latin American tertiary hospital
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Fink, Thais T.

; Marques, Heloisa H.S.

; Gualano, Bruno

; Lindoso, Livia

; Bain, Vera

; Astley, Camilla

; Martins, Fernanda

; Matheus, Denise

; Matsuo, Olivia M.

; Suguita, Priscila

; Trindade, Vitor

; Paula, Camila S.Y.

; Farhat, Sylvia C.L.

; Palmeira, Patricia

; Leal, Gabriela N.

; Suzuki, Lisa

; Odone Filho, Vicente

; Carneiro-Sampaio, Magda

; Duarte, Alberto José S.

; Antonangelo, Leila

; Batisttella, Linamara R.

; Polanczyk, Guilherme V.

; Pereira, Rosa Maria R.

; Carvalho, Carlos Roberto R.

; Buchpiguel, Carlos A.

; Latronico, Ana Claudia

; Seelaender, Marilia

; Silva, Clovis Artur

; Pereira, Maria Fernanda B.

; Sallum, Adriana M. E.

; Brentani, Alexandra V. M.

; Neto, Álvaro José S.

; Ihara, Amanda

; Santos, Andrea R.

; Canton, Ana Pinheiro M.

; Watanabe, Andreia

; Santos, Angélica C. dos

; Pastorino, Antonio C.

; Franco, Bernadette D. G. M.

; Caruzo, Bruna

; Ceneviva, Carina

; Martins, Carolina C. M. F.

; Prado, Danilo

; Abellan, Deipara M.

; Benatti, Fabiana B.

; Smaria, Fabiana

; Gonçalves, Fernanda T.

; Penteado, Fernando D.

; Castro, Gabriela S. F. de

; Gonçalves, Guilherme S.

; Roschel, Hamilton

; Disi, Ilana R.

; Marques, Isabela G.

; Castro, Inar A.

; Buscatti, Izabel M.

; Faiad, Jaline Z.

; Fiamoncini, Jarlei

; Rodrigues, Joaquim C.

; Carneiro, Jorge D. A.

; Paz, Jose A.

; Ferreira, Juliana C.

; Ferreira, Juliana C. O.

; Silva, Katia R.

; Bastos, Karina L. M.

; Kozu, Katia

; Cristofani, Lilian M.

; Souza, Lucas V. B.

; Campos, Lucia M. A.

; Silva Filho, Luiz Vicente R. F.

; Sapienza, Marcelo T.

; Lima, Marcos S.

; Garanito, Marlene P.

; Santos, Márcia F. A.

; Dorna, Mayra B.

; Aikawa, Nadia E.

; Litvinov, Nadia

; Sakita, Neusa K.

; Gaiolla, Paula V. V.

; Pasqualucci, Paula

; Toma, Ricardo K.

; Correa-Silva, Simone

; Sieczkowska, Sofia M.

; Imamura, Marta

; Forsait, Silvana

; Santos, Vera A.

; Zheng, Yingying

Clinics

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2021, Volume 76 elocation e3511

Resumo: En | Texto: En | PDF: En

OBJECTIVES: To prospectively evaluate demographic, anthropometric and health-related quality of life (HRQoL) in pediatric patients with laboratory-confirmed coronavirus disease 2019 (COVID-19) METHODS: This was a longitudinal observational study of surviving pediatric post-COVID-19 patients (n=53) and pediatric subjects without laboratory-confirmed COVID-19 included as controls (n=52) was performed. RESULTS: The median duration between COVID-19 diagnosis (n=53) and follow-up was 4.4 months (0.8-10.7). Twenty-three of 53 (43%) patients reported at least one persistent symptom at the longitudinal follow-up visit and 12/53 (23%) had long COVID-19, with at least one symptom lasting for >12 weeks. The most frequently reported symptoms at the longitudinal follow-up visit were headache (19%), severe recurrent headache (9%), tiredness (9%), dyspnea (8%), and concentration difficulty (4%). At the longitudinal follow-up visit, the frequencies of anemia (11% versus 0%, p=0.030), lymphopenia (42% versus 18%, p=0.020), C-reactive protein level of >30 mg/L (35% versus 0%, p=0.0001), and D-dimer level of >1000 ng/mL (43% versus 6%, p=0.0004) significantly reduced compared with baseline values. Chest X-ray abnormalities (11% versus 2%, p=0.178) and cardiac alterations on echocardiogram (33% versus 22%, p=0.462) were similar at both visits. Comparison of characteristic data between patients with COVID-19 at the longitudinal follow-up visit and controls showed similar age (p=0.962), proportion of male sex (p=0.907), ethnicity (p=0.566), family minimum monthly wage (p=0.664), body mass index (p=0.601), and pediatric pre-existing chronic conditions (p=1.000). The Pediatric Quality of Live Inventory 4.0 scores, median physical score (69 [0-100] versus 81 [34-100], p=0.012), and school score (60 [15-100] versus 70 [15-95], p=0.028) were significantly lower in pediatric patients with COVID-19 at the longitudinal follow-up visit than in controls. CONCLUSIONS: Pediatric patients with COVID-19 showed a longitudinal impact on HRQoL parameters, particularly in physical/school domains, reinforcing the need for a prospective multidisciplinary approach for these patients. These data highlight the importance of closer monitoring of children and adolescents by the clinical team after COVID-19.

https://doi.org/10.6061/clinics/2021/e3511

11.

Activated Complex Approach to Describe Bovine Serum Albumin-Azure A and Bovine Serum Albumin-Azure B Intermolecular Interactions
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Paula, Hauster M. C. de

; Coelho, Yara L.

; Castro, Alan S. B. de

; Rezende, Jaqueline P.

; Pires, Ana C. S.

; Silva, Luis H. M. da

Journal of the Brazilian Chemical Society

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Dez 2020, Volume 31 Nº 12 Páginas 2611 - 2619

Resumo: En | Texto: En | PDF: En

Azure A (AZA) and azure B (AZB) phenothiazine dyes are used for clinical and medical purposes, and their functions can be altered via interactions with proteins. However, no kinetics information on the interactions between phenothiazine dyes and bovine serum albumin (BSA) is available. Surface plasmon resonance was used to determine the energetic and dynamic of the BSA-AZA and BSA-AZB complexes formation at pH 7.4. At temperature ≤ 16 °C, the formation of activated (DH‡a,12°C,AZA= -310.57 kJ mol-1 and DH‡a,12 °C,AZB= -256.37 kJ mol-1) and thermodynamically stable (DH°12°C,AZA= -314.56 kJ mol-1 and DH°12°C,AZB= -265.73 kJ mol-1) complexes was driven by enthalpy, while at temperature ≥ 20 °C, by entropy, (TDS‡a,28°C,AZA= 207.49 and TDS‡a,28°C,AZB= 190.69; TDS°28°C,AZA= 277.50 and TDS°28°C,AZB= 257.26 kJ mol-1). Hydrophobic interactions were fundamental to the complex stability and the increase in number of -CH3 groups in the dyes do not affect kinetic and thermodynamic parameters. Our results could help optimize the medical and pharmaceutical applications of phenothiazine dyes.

https://doi.org/10.21577/0103-5053.20200143 276 downloads

12.

Characteristics and Surgical Outcomes in Very Elderly Patients (≥75 years) with Renal Cell Carcinoma: Data from the Latin American Renal Cancer Group
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Garza-Gangemi, Adrián M.

; Castillejos-Molina, Ricardo A.

; Gueglio, Guillermo

; Tobia-Gonzalez, Ignacio P.

; Jurado, Alberto M.

; Meza-Montoya, Luis

; Scorticati, Carlos H.

; Henriques-da-Costa, Walter

; Yandian, Juan

; Ubillos, Luis

; Glina, Sidney

; Tobias-Machado, Marcos

; Rodríguez-Faba, Oscar

; Ameri, Carlos

; Nolazco, Alejandro

; Martinez, Pablo

; Franco Carvalhal, Gustavo

; Bengio, Ruben G.

; Arribillaga, Leandro Cristian

; Langenhin, Raúl

; Muguruza, Diego

; Campos-Salcedo, José G.

; Bravo-Castro, Edgar I.

; Mingote, Pablo A.

; Ginestar, Nicolás

; Autran-Gomez, Ana M.

; Puente, Roberto

; Decia, Ricardo

; Cardoso-Guimarães, Gustavo

; Palou-Redorta, Joan

; Abreu-Clavijo, Diego

; Cassio-Zequi, Stenio de

; Rodriguez-Covarrubias, Francisco T.

Revista de investigación clínica

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Out 2020, Volume 72 Nº 5 Páginas 308 - 315

Resumo: En | Texto: En | PDF: En

Background: The incidence of renal cell carcinoma (RCC) is increasing globally due to an aging population and widespread use of imaging studies. Objective: The aim of this study was to describe the characteristics and perioperative outcomes of RCC surgery in very elderly patients (VEP), ≥ 75 years of age. Methods: This is a retrospective comparative study of 3656 patients who underwent the treatment for RCC from 1990 to 2015 in 28 centers from eight Latin American countries. We compared baseline characteristics as well as clinical and perioperative outcomes according to age groups (<75 vs. ≥75 years). Surgical complications were classified with the Clavien-Dindo score. We performed logistic regression analysis to identify factors associated with perioperative complications. Results: There were 410 VEP patients (11.2%). On bivariate analysis, VEP had a lower body mass index (p < 0.01) and higher ASA score (ASA >2 in 26.3% vs. 12.4%, p < 0.01). There was no difference in performance status and clinical stage between the study groups. There were no differences in surgical margins, estimated blood loss (EBL), complication, and mortality rates (1.3% vs. 0.4%, p = 0.17). On multivariate regression analysis, age ≥75 years (odds ratio [OR] 2.33, p < 0.01), EBL ≥ 500 cc (OR 3.34, p < 0.01), and > pT2 stage (OR 1.63, p = 0.04) were independently associated with perioperative complications. Conclusions: Surgical resection of RCC was safe and successful in VEP. Age ≥75 years was independently associated with 30-day perioperative complications. However, the vast majority were low-grade complications. Age alone should not guide decision-making in these patients, and treatment must be tailored according to performance status and severity of comorbidities. (REV INVEST CLIN. 2020;72(5):308-15)

https://doi.org/10.24875/ric.20000018 141 downloads

13.

Sanitary quality of seed of Paspalum species
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Silva, Stella Áurea Cristiane Gomes da

; Santos, Andreza Gonçalves dos

; Loges, Vivian

; Castro, Ana Cecília Ribeiro de

; Rosa, Regina Ceres Torres da

Ornamental Horticulture

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Set 2020, Volume 26 Nº 3 Páginas 328 - 332

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo O Brasil vem desenvolvendo pesquisas para seleção de espécies nativas de Paspalum para uso como gramados, porém pouco se sabe sobre a qualidade sanitária e os patógenos associados às sementes, sendo os objetivos deste estudo. Subamostras de 100 sementes de dois acessos de Paspalum notatum Flügge (PN 01 and PN 04) e de um acesso de Paspalum lepton Schult. (PL 01) foram submetidas ao teste de papel de filtro a 20 °C, com fotoperíodo de 12 h, por 7 dias. Os gêneros Penicillium e Curvularia apresentaram maior incidência nos três acessos de Paspalum. A qualidade das sementes foi variável entre os acessos, com mais de 56% de incidência do fungo Curvularia nas sementes. Este estudo relatou pela primeira vez a ocorrência de Curvularia spp. e Cladosporium herbarum em sementes de P. lepton na região nordeste do Brasil.

Abstract Brazil has been developing research for selection of native species of Paspalum for use as turfgrass, nevertheless little is known about the sanitary quality and pathogens associated to the seeds, being the aims of this study. Subsamples of 100 seeds of two accessions of Paspalum notatum Flügge (PN 01 and PN 04) and one accesses of Paspalum lepton Schult. (PL 01) were submitted to the blotter test at 20 °C with a 12-h photoperiod for 7 days. The Penicillium and Curvularia genus incidence were highest in the three accessions of Paspalum. The seed health quality was variable between accessions, with more than 56% of incidence of the fungus Curvularia on the seeds. This study reported for the first time the occurrence of Curvularia spp. and Cladosporium herbarium in seeds of P. lepton, in the Northeast region of Brazil.

https://doi.org/10.1590/2447-536x.v26i3.2065 576 downloads

14.

Selection of Brazilian native turfgrass accessions for establishment by sprigs
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Silva, Stella Áurea Cristiane Gomes da

; Albuquerque Filho, João Carlos Cezar de

; Silva, Simone Santos Lira

; Castro, Ana Cecília Ribeiro de

; Loges, Vivian

Ornamental Horticulture

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Set 2020, Volume 26 Nº 3 Páginas 458 - 467

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo Pesquisas com plantas nativas brasileiras indicam o uso de espécies do gênero Axonopus e Paspalum como gramados. Esta pesquisa avaliou o uso sprigs, que são fragmentos de rizomas e/ou estolões da planta, sem substratos e com parte aérea reduzida, como forma de propagação para implantação de gramados. Dois experimentos foram desenvolvidos. Experimento 1: tapetes de grama (1026 cm²) de acessos de Axonopus parodii (AP 01), Paspalum lepton (PL 01) e P. notatum (PN 01 a PN 06) foram fragmentados em triturador para a obtenção dos sprigs. Depois de removido o substrato e resíduos orgânicos dos sprigs, foi avaliado: número total de sprigs; comprimento mínimo, máximo e médio; desvio padrão; e número de sprigs com comprimento menor que 2 cm; entre 2 e 4 cm; e maior que 4 a 6 cm. Experimento 2: sprigs das três classes de comprimentos foram plantados e avaliados aos 63 dias após o plantio. A variedade comercial Zoysia japonica (ZJ 01) foi utilizada como tratamento controle. Foram avaliadas: taxa de sobrevivência de sprigs; número de perfilhos; expansão; taxa de cobertura do solo; biomassa seca da parte aérea; e biomassa seca radicular. Correlações foram feitas entre os dados. Foram obtidos maior número de sprigs com comprimento menores que 2 cm e de 2 a 4 cm. Os acessos apresentaram melhor desenvolvimento quando estabelecidos com sprigs acima de 2 cm de comprimento. Os acessos de Paspalum notatum PN 01, PN 02, PN 03 e PN 05 podem ser selecionados para o estabelecimento de gramados por sprigs.

Abstract Researches with Brazilian native species of Axonopus and Paspalum genus have indicated their lawn-use. Thus, this research aimed to evaluate the use of sprigs, which are rhizomes and/or stolons fragments of plants without substrate and with reduced aerial part, as a propagation method for turfgrass establishment. Two experiments were developed. Experiment 1: turfgrass sods (1026 cm²) from Axonopus parodii (AP 01), Paspalum lepton (PL 01) and P. notatum (PN 01 to PN 06) accessions, were fragmented in a straw crushing machine in order to obtain the sprigs. After cleaning the substrate and organic residues from the sprigs, the following variables were analyzed: total number of sprigs; minimum, maximum and average length of the sprigs; standard deviation; number of sprigs shorter than 2 cm, from 2 to 4 cm, and from 4 to 6 cm long. Experiment 2: sprigs from three different length classes were planted and evaluated at 63 days after planting. The commercial variety Zoysia japonica (ZJ 01) was used as a control treatment. The following characters were analyzed: the survival rate of the sprigs, the number of shoots, expansion, soil coverage rate, dry biomass of aerial part, and dry biomass of the roots. Correlations were made among the data obtained. Higher yields were obtained for sprigs shorter than 2 cm and from 2 to 4 cm. All accessions presented better development when established with sprigs longer than 2 cm. The Paspalum notatum accessions PN 01, PN 02, PN 03 and PN 05 could be selected for turfgrass establishment by sprigs propagation.

https://doi.org/10.1590/2447-536x.v26i3.2162 455 downloads

15.

Development of Paspalum accession plugs for turfgrass establishment
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Silva, Stella Áurea Cristiane Gomes da

; Albuquerque Filho, João Carlos Cezar de

; Silva, Simone Santos Lira

; Castro, Ana Cecília Ribeiro de

; Loges, Vivian

Ornamental Horticulture

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Set 2020, Volume 26 Nº 3 Páginas 356 - 366

Resumo: Pt En | Texto: Pt En | PDF: Pt | PDF: En

Resumo A seleção de um genótipo para uso como gramado deve considerar a taxa de sobrevivência da planta e a capacidade de cobertura do solo, que está diretamente influenciada pelo crescimento de estolões e rizomas. Este estudo objetivou avaliar o crescimento e desenvolvimento de plugs de acessos de Paspalum para o estabelecimento de gramados. Foram plantados plugs de seis acessos de crescimento prostrado de Paspalum lepton (PL 01), P. notatum (de PN 01 a PN 06) e Zoysia japonica (ZJ 01) como tratamento controle, totalizando de 7 tratamentos. Aos 63 dias após o plantio, foi avaliada a taxa de sobrevivência, número de perfilhos, expansão, altura da planta e taxa de cobertura do solo. Aos 72 dias após o plantio, foi avaliada a biomassa fresca e seca foliar, radicular, de rizoma e/ou estolão e comprimento radicular. Os plugs de PL 01 apresentaram bom crescimento com elevado número de perfilhos, capacidade de expansão, cobertura do solo e bom desenvolvimento do sistema radicular com grande produção de biomassa. PL 01 e PN 05, juntamente com ZJ 01 apresentaram elevada taxa de cobertura do solo e todos os acessos de P. notatum (PN) junto com ZJ 01 mantiveram menor porte da planta, porém com um comprimento do sistema radicular superior ao do tratamento controle ZP 01. Os acessos de P. lepton (PL 01) e P. notatum (PN 05) podem ser recomendados para o estabelecimento de gramado utilizando os métodos de propagação por plugs.

Abstract The selection of a genotype for lawn use must consider the plant survival rate and its soil covering ability, which is directly influenced by the growth of stolons and rhizomes. This study aimed to evaluate the growth and development of Paspalum accession plugs for turfgrass establishment. Plugs of six prostrate growth accessions of Paspalum lepton (PL 01), P. notatum (from PN 01 to PN 06) and Zoysia japonica (ZJ 01), which was used as the control treatment, were planted adding up to 7 treatments. At 63 days after planting, the survival rate, the number of shoots, the expansion capacity, plant height and soil coverage rate were evaluated. Moreover, at 72 days after planting, fresh and dry leaf, root, rhizome and/or stolon biomass, as well as root length were evaluated. Plugs of PL 01 presented the highest values regarding the number of shoots, the expansion capacity, soil coverage and the development of the root system with the largest biomass production. PL 01 and PN 05 along with ZJ 01 presented a high soil coverage rate and all the P. notatum (PN) accessions together with ZJ 01 maintained a lower plant height, but with a length root system higher than the control treatment ZJ 01. The P. lepton (PL 01) and P. notatum (PN 05) accessions can be recommended for turfgrass establishment using plug propagation methods.

https://doi.org/10.1590/2447-536x.v26i3.2163 453 downloads

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